DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP
نویسندگان
چکیده
منابع مشابه
Brain Tissue Cysts in Infected Mice with RH-Strain of Toxoplasma gondii and Evaluation of BAG1 and SAG1 Genes Expression
BACKGROUND Toxoplasma gondii is an obligate intracellular parasite that infects humans at high prevalence rates. The virulent RH strain of T. gondii is generally considered to have lost its cyst forming capacity. This study performed to obtain tissue cysts in mice infected with tachyzoites of RH strain treated with sulfadiazine (SDZ). It provides the opportunity to analyze the conversion of tac...
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A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37 degrees C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the bright...
متن کاملbrain tissue cysts in infected mice with rh-strain of toxoplasma gondii and evaluation of bag1 and sag1 genes expression.
background : toxoplasma gondii is an obligate intracellular parasite that infects humans at high preva-lence rates. the virulent rh strain of t. gondii is generally considered to have lost its cyst forming ca-pacity. this study performed to obtain tissue cysts in mice infected with tachyzoites of rh strain treated with sulfadiazine (sdz). it provides the opportunity to analyze the conversion of...
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Toxoplasmosis is a disease of zoonotic nature; being reported to be widespread in animals and humans. Serological diagnosis represents the first and the most widely used approach to define the stage of toxoplasmosis and diagnosis of primary and late infection in pregnancy can be improved by determination of Toxoplasma DNA. Eighty-eight and 88 coagulated and non coagulated blood samples were col...
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The sensitivity of digoxigenin and biotin labelled DNA probes for the detection of human papillomavirus (HPV) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. Probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and ...
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ژورنال
عنوان ژورنال: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
سال: 2013
ISSN: 2502-5600,1978-225X
DOI: 10.21157/j.ked.hewan.v7i1.579